Chip cube unit and the Chip Cube Interface Assembly (also referred to as
Spray Chamber) and wiring harness with connectors for interface
the invention of HPLC-Chip technology, Agilent is significantly
reducing the need for user interaction and making nanospray a rugged
state-of-the-art technology. The cornerstone of chip technology is the
HLPC-Chip, a 3-dimensional structure made of sandwiched polyimide films.
Grooves of specific dimensions are laser-ablated into a layer of
polyimide film. The lamination of a top and bottom layer then forms
channels of trapezoidal or triangular shape inside the chip which can
either be used as capillaries or, if filled with packing material, as
nanocolumns. One end of the chip tapers off into a polyimide
The Chip Cube
belongs to the Agilent 1260 Infinity HPLC family. It interfaces the
HPLC-Chip to the pump and the μ-well-plate sampler of the nano-LC
system. The Chip Cube is connected to the other Agilent 1260 Infinity
modules via CAN bus. The main functions are:
• Connect the HPLC-Chip automatically to solvent lines and grounding contact. • Position the sprayer tip reproducible in front of the MS inlet. • Provide two independent, concentrically high pressure nano flow valves.
The Chip Cube Interface Assembly
The Chip Cube Interface Assembly is effectively a spray chamber for use with the Chip Cube.